IL-32 Expression Analyzed in Chronic Rhinosinusitis
Expressed differently in chronic rhinosinusitis with and without nasal polyps
THURSDAY, Dec. 15 (HealthDay News) -- In patients with nonpolypoid chronic rhinosinusitis (CRSsNP), interleukin (IL)-32 expression is elevated in nasal epithelial cells, while those with polypoid chronic rhinosinusitis (CRSwNP) show increases in IL-32-producing macrophages and T cells, according to research published in the January issue of Allergy.
Anjeni Keswani, M.D., of the Northwestern University Feinberg School of Medicine in Chicago, and colleagues analyzed nasal epithelial cells and nasal tissue from 94 adults with CRS (54 with CRSwNP and 40 with CRSsNP) as well as 24 inflammation-free controls. The samples were assayed for IL-32 mRNA expression by real-time polymerase chain reaction; IL-32 protein was measured by immunohistochemistry, enzyme-linked immunosorbent assay, and Western blot testing.
According to the researchers, CRSsNP patients had higher IL-32 mRNA expression in the epithelial cells of their uncinate tissue, and significantly higher protein expression in whole tissue, than CRSwNP patients or controls. IL-32 mRNA expression was also higher in epithelial cells of CRSsNP samples compared to nasal polyp epithelial cells. In primary airway epithelial cells, interferon-γ, tumor necrosis factor, and dsRNA were shown to induce IL-32 expression. In nasal polyps, IL-32 expression correlated with CD3 and mannose receptor levels and was shown to be co-localized with CD3+ T cells and CD68+ macrophages.
"In summary, we report here that IL-32 is elevated in epithelial cells from patients with CRSsNP and that IL-32-producing T cells and macrophages are elevated in nasal tissue from patients with CRSwNP," the authors write. "These results support the conclusion that the role of IL-32 in inflammation associated with CRSsNP and CRSwNP may be different."