Plant Fibers May Play Role in Crohn's Pathogenesis
Plantain, broccoli fibers found to inhibit E. coli translocation across microfold cells
THURSDAY, Sept. 2 (HealthDay News) -- Plantain and broccoli soluble plant fibers inhibit the translocation of Escherichia coli (E. coli) across microfold epithelial cells (M-cells) and Peyer's patches, but an emulsifier in many processed foods increases it, according to research published online Sept. 2 in Gut.
Carol L. Roberts, of the University of Liverpool in the United Kingdom, and colleagues analyzed data from experiments using M-cell monolayers created by culturing human colorectal adenocarcinoma cell-line Caco2-cl1 with Raji B cells, as well as experiments with human Peyer's patches.
The researchers found that translocation of E. coli was greater across M-cells versus parent Caco2-cl1 monocultures. At 5 mg/mL, plantain and broccoli non-starch polysaccharide (NSP) steeply inhibited E. coli translocation across M-cells by 45.3 to 82.6 percent. However, apple and leek NSP didn't have a significant effect. Polysorbate-80 -- a common emulsifier in processed food -- was associated with increased translocation across M-cells. E. coli translocation across human Peyer's patches was also reduced with soluble plantain NSP and increased with polysorbate-80.
"These studies show that different dietary components may have powerful and contrasting effects on bacterial translocation across intestinal M-cells. These effects may be relevant to the role of environmental factors in the pathogenesis of Crohn's disease and suggest possible novel therapeutic approaches," the authors conclude.
Two co-authors disclosed financial relationships with the pharmaceutical industry, and one author holds a patent related to the subject of this study.