H. pylori Not Detected in Hyperplastic Adenoids
H. pylori, other Helicobacteraceae family members not major cause of enlarged adenoids in children
WEDNESDAY, Oct. 19 (HealthDay News) -- Helicobacter pylori (H. pylori) and other Helicobacteraceae family members do not majorly contribute toward development of hyperplastic adenoids in children, according to a study published in the October issue of the Archives of Otolaryngology -- Head & Neck Surgery.
Damian J. Hussey, Ph.D., from the Flinders Medical Centre and Flinders University in Adelaide, Australia, and colleagues investigated whether H. pylori and/or other members of the Helicobacteraceae family were detected in hyperplastic adenoids of children, and whether false-positive H. pylori detection has been reported by previous polymerase chain reaction (PCR) methods. Total RNA was extracted before reverse transcription of bacterial RNA from 78 hyperplastic and 15 normal children aged 2 to 10 years. Each biopsy sample was histologically examined, and a nested reverse transcription-PCR protocol was designed for identification of all species of the Helicobacteraceae family.
The investigators found that, on the basis of the Reflux Symptom Index, laryngopharyngeal reflux was suspected in 41 percent of the children. No adenoid sample had any evidence of H. pylori, while one hyperplastic adenoid sample showed Candidatus Wolinella africanus, the only Helicobacteraceae family member detected. Very few bacterial organisms were found on histological examination. Previous H. pylori reports in hyperplastic adenoids of children may have resulted from false-positive PCR results.
"Inflammation and enlargement of the adenoids is not likely due to ongoing bacterial infection arising from laryngopharyngeal reflux. We conclude that H. pylori and other Helicobacteraceae family members are not major contributors to the development of hyperplastic adenoids in children," the authors write.